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flag krt19 overexpression  (Proteintech)


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    Proteintech flag krt19 overexpression
    KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from <t>Flag-KRT19-overexpressing</t> A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)
    Flag Krt19 Overexpression, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flag krt19 overexpression/product/Proteintech
    Average 96 stars, based on 2110 article reviews
    flag krt19 overexpression - by Bioz Stars, 2026-03
    96/100 stars

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    1) Product Images from "Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence"

    Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-025-03602-5

    KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from Flag-KRT19-overexpressing A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)
    Figure Legend Snippet: KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from Flag-KRT19-overexpressing A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)

    Techniques Used: Expressing, SDS Page, Silver Staining, Immunoprecipitation, Control, Binding Assay, Mass Spectrometry, Co-Immunoprecipitation Assay, Transfection, Plasmid Preparation, Transduction, Immunofluorescence, Western Blot

    KRT19 facilitates p21 ubiquitination at K16 via MYH9 A PC-9 cells were transfected with MYH9 siRNA (siMYH9) or control siRNA (si-NC). The protein levels of p21, p53 and MYH9 in PC-9 cells (si-NC, siMYH9) treated with CHX (15 µg/mL) for the indicated time were measured by immunoblot (Top), and quantitation of p21 protein levels based on band intensity was shown (bottom) B p21 and MYH9 protein expression in PC-9 cells (si-NC, siMYH9) treated with or without MG132 (20 µM) for 4 h was determined by immunoblot C Co-IP analysis of the interaction between p21 and ubiquitin (Ub) in PC-9 cells (si-NC, siMYH9) treated with MG132 (20 µM) for 4 h D HEK293T cells were co-transfected with Myc-p21, HA-Ub and MYH9-overexpressing (MYH9-OVE) plasmids or control plasmid. Co-IP analysis of the interaction between Myc-p21 and HA-Ub in different groups of HEK293T cells treated with MG132 (20 µM) for 4 h E Control or Flag-KRT19-overexpressing (Flag-KRT19) HEK293T cells were co-transfected with siMYH9 or si-NC, Myc-p21 and HA-Ub plasmids. F-G Prediction of ubiquitination sites of p21 protein using GPS-Uber H-I KRT19-Flag-OVE (H) or MYH9-OVE (I) HEK293T cells were co-transfected with HA-Ub and Myc-p21 (WT, K16R, K75R, K154R). The interaction between Myc-p21 and HA-Ub was determined by Co-IP after treatment with MG132 (20 µM) for 4 h J Sequence comparison around the K16 residue (red) of the p21 homologue in different species K-L PC-9 cells were transduced with shKRT19#2 or transfected with MYH9-OVE plasmid for 48 h. p21, KRT19, MYH9 protein expression levels (K) and SA-β-gal staining (L) of different groups of PC-9 cells were shown. Scale bar, 10 μm. Data are shown as mean ± S.E.M. and analyzed by one-way ANOVA (K-L). * p < 0.05; ** p < 0.01; *** p < 0.001. The experiments (A-E, H-I, K-L) were repeated three times
    Figure Legend Snippet: KRT19 facilitates p21 ubiquitination at K16 via MYH9 A PC-9 cells were transfected with MYH9 siRNA (siMYH9) or control siRNA (si-NC). The protein levels of p21, p53 and MYH9 in PC-9 cells (si-NC, siMYH9) treated with CHX (15 µg/mL) for the indicated time were measured by immunoblot (Top), and quantitation of p21 protein levels based on band intensity was shown (bottom) B p21 and MYH9 protein expression in PC-9 cells (si-NC, siMYH9) treated with or without MG132 (20 µM) for 4 h was determined by immunoblot C Co-IP analysis of the interaction between p21 and ubiquitin (Ub) in PC-9 cells (si-NC, siMYH9) treated with MG132 (20 µM) for 4 h D HEK293T cells were co-transfected with Myc-p21, HA-Ub and MYH9-overexpressing (MYH9-OVE) plasmids or control plasmid. Co-IP analysis of the interaction between Myc-p21 and HA-Ub in different groups of HEK293T cells treated with MG132 (20 µM) for 4 h E Control or Flag-KRT19-overexpressing (Flag-KRT19) HEK293T cells were co-transfected with siMYH9 or si-NC, Myc-p21 and HA-Ub plasmids. F-G Prediction of ubiquitination sites of p21 protein using GPS-Uber H-I KRT19-Flag-OVE (H) or MYH9-OVE (I) HEK293T cells were co-transfected with HA-Ub and Myc-p21 (WT, K16R, K75R, K154R). The interaction between Myc-p21 and HA-Ub was determined by Co-IP after treatment with MG132 (20 µM) for 4 h J Sequence comparison around the K16 residue (red) of the p21 homologue in different species K-L PC-9 cells were transduced with shKRT19#2 or transfected with MYH9-OVE plasmid for 48 h. p21, KRT19, MYH9 protein expression levels (K) and SA-β-gal staining (L) of different groups of PC-9 cells were shown. Scale bar, 10 μm. Data are shown as mean ± S.E.M. and analyzed by one-way ANOVA (K-L). * p < 0.05; ** p < 0.01; *** p < 0.001. The experiments (A-E, H-I, K-L) were repeated three times

    Techniques Used: Ubiquitin Proteomics, Transfection, Control, Western Blot, Quantitation Assay, Expressing, Co-Immunoprecipitation Assay, Plasmid Preparation, Sequencing, Comparison, Residue, Transduction, Staining



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    flag krt19 overexpression  (Proteintech)
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    Proteintech flag krt19 overexpression
    KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from <t>Flag-KRT19-overexpressing</t> A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)
    Flag Krt19 Overexpression, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flag krt19 overexpression/product/Proteintech
    Average 96 stars, based on 1 article reviews
    flag krt19 overexpression - by Bioz Stars, 2026-03
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    		  Buy from Supplier

    Image Search Results


    KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from Flag-KRT19-overexpressing A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence

    doi: 10.1186/s13046-025-03602-5

    Figure Lengend Snippet: KRT19 interacts with MYH9 and induces its expression A Schematic representation of experimental setup for identifying KRT19-interacting proteins B Representative SDS-PAGE separation and silver staining of protein lysates from Flag-KRT19-overexpressing A549 cells immunoprecipitated with anti-Flag or control IgG C List of potential KRT19-binding proteins identified by mass spectrometry D-E The interaction of endogenous KRT19 and MYH9 in A549 cells was determined by Co-IP F-I A549 (F-G) and HEK293T cells (H-I) were transfected with MYH9-overexpressing plasmid and transduced with Flag-KRT19 lentivirus (Flag-KRT19 group) or control empty vector (EV group). Representative immunofluorescence images of A549 (F) and HEK293T cells (H), and co-localization analysis of Flag and MYH9 in Flag-KRT19 A549 (G) and HEK293T cells (I) were shown. Scale bar, 10 μm J-K KRT19 and MYH9 protein levels in different groups of A549 cells (J) and PC-9 cells (K) were measured by immunoblot. Data are shown as mean ± S.E.M. and analyzed by Student’s t -test (J-K). * p < 0.05; ** p < 0.01. The experiments were repeated two (A-C) or three times (D-K)

    Article Snippet: To identify the proteins interacted with KRT19, total extracts of A549 cells with stable Flag-KRT19 overexpression were immunoprecipitated with Flag tag antibody (66008-4-Ig, Proteintech) or control IgG as previously described, and subjected to SDS-PAGE.

    Techniques: Expressing, SDS Page, Silver Staining, Immunoprecipitation, Control, Binding Assay, Mass Spectrometry, Co-Immunoprecipitation Assay, Transfection, Plasmid Preparation, Transduction, Immunofluorescence, Western Blot

    KRT19 facilitates p21 ubiquitination at K16 via MYH9 A PC-9 cells were transfected with MYH9 siRNA (siMYH9) or control siRNA (si-NC). The protein levels of p21, p53 and MYH9 in PC-9 cells (si-NC, siMYH9) treated with CHX (15 µg/mL) for the indicated time were measured by immunoblot (Top), and quantitation of p21 protein levels based on band intensity was shown (bottom) B p21 and MYH9 protein expression in PC-9 cells (si-NC, siMYH9) treated with or without MG132 (20 µM) for 4 h was determined by immunoblot C Co-IP analysis of the interaction between p21 and ubiquitin (Ub) in PC-9 cells (si-NC, siMYH9) treated with MG132 (20 µM) for 4 h D HEK293T cells were co-transfected with Myc-p21, HA-Ub and MYH9-overexpressing (MYH9-OVE) plasmids or control plasmid. Co-IP analysis of the interaction between Myc-p21 and HA-Ub in different groups of HEK293T cells treated with MG132 (20 µM) for 4 h E Control or Flag-KRT19-overexpressing (Flag-KRT19) HEK293T cells were co-transfected with siMYH9 or si-NC, Myc-p21 and HA-Ub plasmids. F-G Prediction of ubiquitination sites of p21 protein using GPS-Uber H-I KRT19-Flag-OVE (H) or MYH9-OVE (I) HEK293T cells were co-transfected with HA-Ub and Myc-p21 (WT, K16R, K75R, K154R). The interaction between Myc-p21 and HA-Ub was determined by Co-IP after treatment with MG132 (20 µM) for 4 h J Sequence comparison around the K16 residue (red) of the p21 homologue in different species K-L PC-9 cells were transduced with shKRT19#2 or transfected with MYH9-OVE plasmid for 48 h. p21, KRT19, MYH9 protein expression levels (K) and SA-β-gal staining (L) of different groups of PC-9 cells were shown. Scale bar, 10 μm. Data are shown as mean ± S.E.M. and analyzed by one-way ANOVA (K-L). * p < 0.05; ** p < 0.01; *** p < 0.001. The experiments (A-E, H-I, K-L) were repeated three times

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence

    doi: 10.1186/s13046-025-03602-5

    Figure Lengend Snippet: KRT19 facilitates p21 ubiquitination at K16 via MYH9 A PC-9 cells were transfected with MYH9 siRNA (siMYH9) or control siRNA (si-NC). The protein levels of p21, p53 and MYH9 in PC-9 cells (si-NC, siMYH9) treated with CHX (15 µg/mL) for the indicated time were measured by immunoblot (Top), and quantitation of p21 protein levels based on band intensity was shown (bottom) B p21 and MYH9 protein expression in PC-9 cells (si-NC, siMYH9) treated with or without MG132 (20 µM) for 4 h was determined by immunoblot C Co-IP analysis of the interaction between p21 and ubiquitin (Ub) in PC-9 cells (si-NC, siMYH9) treated with MG132 (20 µM) for 4 h D HEK293T cells were co-transfected with Myc-p21, HA-Ub and MYH9-overexpressing (MYH9-OVE) plasmids or control plasmid. Co-IP analysis of the interaction between Myc-p21 and HA-Ub in different groups of HEK293T cells treated with MG132 (20 µM) for 4 h E Control or Flag-KRT19-overexpressing (Flag-KRT19) HEK293T cells were co-transfected with siMYH9 or si-NC, Myc-p21 and HA-Ub plasmids. F-G Prediction of ubiquitination sites of p21 protein using GPS-Uber H-I KRT19-Flag-OVE (H) or MYH9-OVE (I) HEK293T cells were co-transfected with HA-Ub and Myc-p21 (WT, K16R, K75R, K154R). The interaction between Myc-p21 and HA-Ub was determined by Co-IP after treatment with MG132 (20 µM) for 4 h J Sequence comparison around the K16 residue (red) of the p21 homologue in different species K-L PC-9 cells were transduced with shKRT19#2 or transfected with MYH9-OVE plasmid for 48 h. p21, KRT19, MYH9 protein expression levels (K) and SA-β-gal staining (L) of different groups of PC-9 cells were shown. Scale bar, 10 μm. Data are shown as mean ± S.E.M. and analyzed by one-way ANOVA (K-L). * p < 0.05; ** p < 0.01; *** p < 0.001. The experiments (A-E, H-I, K-L) were repeated three times

    Article Snippet: To identify the proteins interacted with KRT19, total extracts of A549 cells with stable Flag-KRT19 overexpression were immunoprecipitated with Flag tag antibody (66008-4-Ig, Proteintech) or control IgG as previously described, and subjected to SDS-PAGE.

    Techniques: Ubiquitin Proteomics, Transfection, Control, Western Blot, Quantitation Assay, Expressing, Co-Immunoprecipitation Assay, Plasmid Preparation, Sequencing, Comparison, Residue, Transduction, Staining